GENOTOXICITY OF CERIUM OXIDE NANOPARTICLE IN ZEBRAFISH AND GREEN MUSSEL PERNA VIRIDIS USING ALKALINE COMET ASSAY

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GENOTOXICITY OF CERIUM OXIDE NANOPARTICLE IN ZEBRAFISH AND GREEN MUSSEL PERNA VIRIDIS USING ALKALINE COMET ASSAY

Jacky Bhagat

Department of Zoology, Goa University, Taleigao Plateau, Goa, India

bhagatjack@gmail.com

Greeshma S S

Demphe College of Arts and Science, Miramar, Goa-403001, India

greeshmasoorambail@gmail.com

S K Shyama

Department of Zoology, Goa University, Taleigao Plateau, Goa-403206, India

skshyama@gmail.com

Cerium oxide nanoparticles or nanoceria has versatile application in biomedical, solar cells and gas sensors. Increasing utilization of nanoceria has raised concerns over its release to the environment and potential exposure. In vitro studies have shown its genotoxic potential, but reports on aquatic life are very limited. In this study, zebrafish (Danio rerio) and green mussel (Perna viridis) was exposed to different concentration 10, 20, 50 µg/l of nanoceria for 24, 72, and 120 h and the genotoxic response was measured using comet assay. The results showed significant (p < 0.05) increase in tail DNA (TDNA) and olive tail moment (OTM) as measured using comet assay in exposed animals as compared to control. The highest TDNA and OTM were measured after 120 h of exposure with 50 µg/l of nanoceria in zebrafish as well as in green mussel. The results of this study demonstrate that short-term exposure to nanoceria causes a genotoxic response in zebrafish and green mussel, hence its environmental release should be carefully monitored.

Keywords

Single Cell Gel Electrophoresis, Nano ceria, DNA Damage, Zebra fish, Green Mussel

 

Full Article available at https://dx.doi.org/10.20319/lijhls.2019.43.118127

2019-10-29T19:46:03+00:00
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